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김재우(생화학-분자생물학교실) Sci Rep
등록일 : 2017-11-06 오전 10:38:00       조회 : 75
첨   부 :
제1저자 이혜민(생화학-분자생물학교실, 연세대 융합오믹스의생명과학)
교신저자 김효정 김재우(생화학-분자생물학교실)

Sci Rep. 2017 Nov 1ɕ(1):14833. doi: 10.1038/s41598-017-12512-2.
SCARA5 plays a critical role in the commitment of mesenchymal stem cells to adipogenesis.
Lee H1,2, Lee YJ3, Choi H1, Seok JW1,4, Yoon BK1,4, Kim D1,4, Han JY1,4, Lee Y1,4, Kim HJ5, Kim JW6,7,8.

Author information
1Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, 120-752, Korea.
2Department of Integrated OMICS for Biomedical Sciences, Graduate School, Yonsei University, Seoul, 120-749, Korea.
3Division of Metabolic Disease, Center for Biomedical Sciences, National Institutes of Health, Cheongju-si, Chungbuk, 28159, Korea.
4Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, Korea.
5Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, 120-752, Korea. hjkim17@yuhs.ac.
6Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, 120-752, Korea. japol13@yuhs.ac.
7Department of Integrated OMICS for Biomedical Sciences, Graduate School, Yonsei University, Seoul, 120-749, Korea. japol13@yuhs.ac.
8Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, 120-752, Korea. japol13@yuhs.ac.

Abstract
Mesenchymal stem cells have the capacity to give rise to multiple cell types, such as adipocytes, osteoblasts, chondrocytes, and myocytes. However, the molecular events responsible for the lineage specification and differentiation of mesenchymal stem cells remain unclear. Using gene expression profile studies, we determined that Scavenger receptor class A, member 5 (SCARA5) is a novel mediator of adipocyte commitment. SCARA5 was expressed at a higher level in committed A33 preadipocyte cells compared to C3H10T1/2 pluripotent stem cells. Gain- and loss-of-function studies likewise revealed that SCARA5 acts as a mediator of adipocyte commitment and differentiation in both A33 and C3H10T1/2 cells. RNAi-mediated knockdown of SCARA5 in A33 cells markedly inhibited the adipogenic potential, whereas overexpression of SCARA5 enhanced adipocyte differentiation in C3H10T1/2 cells. We also demonstrated that the focal adhesion kinase (FAK) and ERK signaling pathways is associated with the SCARA5-mediated response, thereby modulating adipocyte lineage commitment and adipocyte differentiation. Additionally, glucocorticoids induced the expression of SCARA5 in differentiating adipocytes through glucocorticoids response elements (GRE) in the SCARA5 promoter. Taken together, our study demonstrates that SCARA5 is a positive regulator in adipocyte lineage commitment and early adipogenesis in mesenchymal stem cells.
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