Home Contact 사이트맵
 
 
HOME > 커뮤니티 > 우수논문소식
최성미(정형외과학교실) Cell Death Dis
등록일 : 2018-09-27 오전 11:15:00       조회 : 267
첨   부 :
제1저자 최성미(정형외과학교실, BK21)
교신저자 이진우(정형외과학교실)

Cell Death Dis. 2018 Aug 29ɗ(9):866. doi: 10.1038/s41419-018-0914-1.
Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel.
Choi SM1,2, Lee KM1,3, Ryu SB4, Park YJ5, Hwang YG1, Baek D1,2, Choi Y1,2, Park KH1, Park KD4, Lee JW6,7,8.

Author information
1Department of Orthopaedic Surgery, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.2Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.3Severance Biomedical Science Institute, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.4Department of Molecular Science and Technology/Applied Chemistry and Biological Engineering, Ajou University, 206, World cup-ro Yeongtong-gu, Suwon-si, Gyeonggi-do, 16499, South Korea.5Department of Orthopaedic Surgery, Yonsei University Wonju College of Medicine, 20, Ilsan-ro, Wonju-si, Gangwon-do, 26426, South Korea.6Department of Orthopaedic Surgery, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea. ljwos@yuhs.ac.7Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea. ljwos@yuhs.ac.8Severance Biomedical Science Institute, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea. ljwos@yuhs.ac.

Abstract
To investigate the functional effects of resveratrol (RSV) on mesenchymal stem cells (MSCs), we treated MSCs with RSV continuously during ex vivo expansion. MSCs were continuously treated with RSV from passage (P) 0 to P5. A proliferative capacity of RSV-treated MSCs was higher than that of non-treated MSCs and similar with P1-MSCs. Continuous treatment of RSV on MSCs increased the stemness and inhibited the senescence. During chondrogenic differentiation in vitro, RSV-treated MSCs had higher differentiation potential and reduced hypertrophic maturation, which are limitations for hyaline cartilage formation. The histological analysis of micromass demonstrated increased chondrogenic differentiation potential. We further explored the therapeutic effectiveness of this method in a rabbit osteochondral defect model. A rabbit osteochondral defect model was established to investigate the hyaline cartilage regeneration potential of RSV-treated MSCs. Moreover, the cartilage regeneration potential of RSV-treated MSCs was greater than that of untreated MSCs. The expression levels of chondrogenic markers increased and those of hypertrophic markers decreased in RSV-treated MSCs compared with untreated MSCs. Sustained treatment of RSV on MSCs during ex vivo expansion resulted in the maintenance of stemness and enhanced chondrogenic differentiation potential. Consequentially, highly efficient MSCs promoted superior hyaline cartilage regeneration in vivo. This novel treatment method provides a basis for cell-based tissue engineering.
양미정 남기택(의생명과학부) Am J Pathol 2018-09-27
엄선우 이배환(생리학교실) Mol Neurobiol 2018-09-09