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양정민 유제욱(미생물학교실) Front Immunol
등록일 : 2017-04-15 오후 2:09:00       조회 : 139
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제1저자 양정민
교신저자 유제욱

Front Immunol. 2017 Mar 27ɖ:333. doi: 10.3389/fimmu.2017.00333. eCollection 2017.

Bacterial Secretant from Pseudomonas aeruginosa Dampens Inflammasome Activation in a Quorum Sensing-Dependent Manner.

Yang J1, Lee KM1, Park S1, Cho Y2, Lee E1, Park JH3, Shin OS4, Son J5, Yoon SS1, Yu JW1.

Author information

1Department of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine , Seoul , South Korea.2Department of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea; Doping Control Center, Korea Institute of Science and Technology, Seoul, South Korea.3Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project Team, Chonnam National University , Gwangju , South Korea.4Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital , Seoul , South Korea.5Doping Control Center, Korea Institute of Science and Technology , Seoul , South Korea.
Abstract

Inflammasome signaling can contribute to host innate immune defense against bacterial pathogens such as Pseudomonas aeruginosa. However, bacterial evasion of host inflammasome activation is still poorly elucidated. Quorum sensing (QS) is a bacterial communication mechanism that promotes coordinated adaptation by triggering expression of a wide range of genes. QS is thought to strongly contribute to the virulence of P. ;aeruginosa, but the molecular impact of bacterial QS on host inflammasome defense is completely unknown. Here, we present evidence that QS-related factors of the bacterial secretant (BS) from P. aeruginosa can dampen host inflammasome signaling in mouse bone marrow-derived macrophages. We found that BS from QS-defective ΔlasR/rhlR mutant, but not from wild-type (WT) P. aeruginosa, induces robust activation of the NLRC4 inflammasome. P. aeruginosa-released flagellin mediates this inflammasome activation by ΔlasR/rhlR secretant, but QS-regulated bacterial proteases in the WT BS impair extracellular flagellin to attenuate NLRC4 inflammasome activation. P. aeruginosa-secreted proteases also degrade inflammasome components in the extracellular space to inhibit the propagation of inflammasome-mediated responses. Furthermore, QS-regulated virulence factor pyocyanin and QS autoinducer 3-oxo-C12-homoserine lactone directly suppressed NLRC4- and even NLRP3-mediated inflammasome assembly and activation. Taken together, our data indicate that QS system of P. aeruginosa facilitates bacteria to evade host inflammasome-dependent sensing machinery.
서동혁 Xiumei Che(내과학교실) Sci Rep 2017-04-18
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