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Mei-yan Wu(내과학교실) J Am Soc Nephrol
등록일 : 2017-08-07 오후 4:07:00       조회 : 31
첨   부 :
제1저자 한승혁(내과학교실) Mei-yan Wu(내과학교실, BK21 지도교수 강신욱)
교신저자 Susztak K(UPENN)

J Am Soc Nephrol. 2017 Jul 27. pii: ASN.2017020130. doi: 10.1681/ASN.2017020130. [Epub ahead of print]
PGC-1α Protects from Notch-Induced Kidney Fibrosis Development.
Han SH1,2, Wu MY3,4, Nam BY3, Park JT1, Yoo TH1, Kang SW1,3, Park J2, Chinga F2, Li SY2, Susztak K5.

Author information
1Department of Internal Medicine, Institute of Kidney Disease Research, Yonsei University College of Medicine, Seoul, Korea.2Renal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.3Severance Biomedical Science Institute, Brain Korea 21 PLUS, Yonsei University College of Medicine, Seoul, Korea; and.4Department of Nephrology, The First Hospital of Jilin University, Changchun, China.5Renal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania; ksusztak@mail.med.upenn.edu.

Kidney fibrosis is the histologic manifestation of CKD. Sustained activation of developmental pathways, such as Notch, in tubule epithelial cells has been shown to have a key role in fibrosis development. The molecular mechanism of Notch-induced fibrosis, however, remains poorly understood. Here, we show that, that expression of peroxisomal proliferation g-coactivator (PGC-1α) and fatty acid oxidation-related genes are lower in mice expressing active Notch1 in tubular epithelial cells (Pax8-rtTA/ICN1) compared to littermate controls. Chromatin immunoprecipitation assays revealed that the Notch target gene Hes1 directly binds to the regulatory region of PGC-1α Compared with Pax8-rtTA/ICN1 transgenic animals, Pax8-rtTA/ICN1/Ppargc1a transgenic mice showed improvement of renal structural alterations (on histology) and molecular defect (expression of profibrotic genes). Overexpression of PGC-1α restored mitochondrial content and reversed the fatty acid oxidation defect induced by Notch overexpression in vitro in tubule cells. Furthermore, compared with Pax8-rtTA/ICN1 mice, Pax8-rtTA/ICN1/Ppargc1a mice exhibited improvement in renal fatty acid oxidation gene expression and apoptosis. Our results show that metabolic dysregulation has a key role in kidney fibrosis induced by sustained activation of the Notch developmental pathway and can be ameliorated by PGC-1α.
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